GSM6610463: V. cholerae Δtgt + tobramycin_replicate 2 [tgttob2_S50]; ... - SRA

SRX17761043: GSM6610463: V. cholerae Δtgt + tobramycin_replicate 2 [tgttob2_S50]; Vibrio cholerae O1 biovar El Tor str. N16961; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 20M spots, 1.4G bases, 462Mb downloads

External Id: GSM6610463_r1

Submitted by: Plasticité du Génome Bactérien, Génomes et Génétique, Institut Pasteur

Study: V. cholerae transcriptome or deleted for the tgt gene in MH, supplemented or not with tobramycin or ciprofloxacin

PRJNA885798 • SRP400425 • All experiments • All runs

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We study gene expression levels of V. cholerae N16961 WT and tgt mutant strains in MH medium in the presence or not of sublethal concentration on tobramycin or ciprofloxacin Overall design: Bacterial cells were grown to OD 0,3 and RNAseq was performed.

Sample: V. cholerae Δtgt + tobramycin_replicate 2 [tgttob2_S50]

SAMN31104805 • SRS15288405 • All experiments • All runs

Organism: Vibrio cholerae O1 biovar El Tor str. N16961

Library:

Name: GSM6610463

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Cultures were centrifuged, and RNA was harvested using Trizol reagent. Illumina TruSeq mRNA Sample Prep Kit (20020595) was used with 7 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols (Krin et al., 2018, PMID: 29783948)

Runs: 1 run, 20M spots, 1.4G bases, 462Mb

Run	# of Spots	# of Bases	Size	Published
SRR21765953	20,014,139	1.4G	462Mb	2022-10-03

ID:: 24669243

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